GI-1 gel imaging system operation guide
GI-1 gel imaging system from the top to the bottom of the upper part of the lens, the aperture - Adjust the image brightness. Zoom - Adjust the size of the image. Focus - Adjust the sharpness of the image
The GI-1 gel imaging system:
WL--- The white light lamp switch.
300--- The UV light transmission switch, used for agarose gel electrophoresis.
254--- UV light reflection switch. For thin layer electrophoresis, dot blot hybridization.
365--- UV light reflection switch. For thin layer electrophoresis, dot blot hybridization.
White: white light transmission conversion board such as light source, the white board on the uviol open 300- UV transmission light switch. But for polypropylene amide electrophoresis
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GI-1 Gel imaging system
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brief introduction
GI-1 gel imaging system is simple and easy to use, real-time gel imaging system with high performance. Through the gel-pro software automatic control for GI-1, we can on the protein gel, DNA gel, 96 hole cell plate, plate samples for image acquisition and analysis.
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The performance of the instrument
1. with high resolution One 280 x 1024 Real time image acquisition CCD
The 2. motor automatic control F/1.2, C- Bayonet lens. In the instrument panel or the software to use the mouse to conveniently control the aperture size ( Iris Zoom lens). (zoom) The depth of focus. (focus) . C- Bayonet, F/1.2 To ensure the The amount of light and high imaging speed.
3.PC Machine controls the opening and closing of light source
The sealing chamber 4. with special design, can be used to upgrade the sealing layer of chemiluminescence signal acquisition
5.GI-1 has a variety of side light source: white light, UV transmission , The white light transmission (Bai Guangping). The side white light for transparent samples such as photo paper, hybrid membrane; UV fluorescence in samples such as transmission EB Dyeing DNA Gel: Set & Match， SYPRO Orange Fluorescent staining DNA , Radiant Red Fluorescent staining RNA Gel; white light transmission is suitable for various dyeing such as protein gel staining, silver staining. And Three Which kind of UV light is optional, to adapt to the user's special needs, they are to 254nm, 3 00nm, 365nm Wide wavelength ultraviolet light source as the center.
The 6. largest plastic area: Two 5 x 20 cm, Suitable for various sizes of gel.
7. preparation UV Light mode ( Prep UV ) and box drawer to facilitate the rubber cutting operation
8.GI-1 supporting software and 1D 2D Software Software, automatic control of the software system, and provides a powerful function of qualitative, quantitative analysis. And image sharing platform, user without password restrictions can be installed and used.
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Radiant Red dyeing RNA? SYPRO Orange dyeing DNA EB dyeing
Performance index:
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CCD (resolution H x V )
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One Twenty-eight 0 x 1024
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Pixel density
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Eight (a Four thousand and ninety-six Gray)
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Automatic zoom lens
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C-mount, f/1.2,8 -48 Mm
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Lighting mode
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UV transmission, transmission of white light, white light
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The excitation light source
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Three 00 And white (standard), Two hundred and fifty-four , 365nm
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Filter location
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One
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Chemiluminescence
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nothing
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Transmission area
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Two Five X 2 Zero CM
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Operating System compatibility
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Windows 2000/xp
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weight
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Twenty Kg
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Gel-pro analysis software is introduced
Software analysis function
It is flexible and a powerful software package, image analysis of one-dimensional gel electrophoresis, intake and dot blot, slot blot and colony counting etc.. For gel bands analysis, microplate and cloning. The results include: DNA/RNA Protein molecular weight and isoelectric point, migration, differential display, RFLP Analysis、 Fingerprinting Analysis and autoradiography film imaging, Ninety-six Quantitative analysis, orifice plate count and double counting cloning.
Stable image acquisition
The application based acquisition control window, can automatically configure the appropriate filter for the imaging system and the light source hardware configuration
Flexible lane and band analysis
The automatic detection of lanes and bands
Fast determination of molecular weight, calculation method of regression calculation statistical multiple regression using a variety of optional mode, calculation of molecular weight.
The band / Matching analysis of lane, generate a tree
Fast and simple quantitative
The use of quantitative tools for advanced, rectangular, round, irregular or arbitrary contour within the region for accurate concentration analysis
O for the individual bands of local background deduction function; automatically records the analysis function parameters, simplifies the analysis of Gauss model of precise and quantitative part of the sample overlapping spots
O the software can automatically, and the analysis of samples appear weak.
The automatic identification in the samples of saturated pixels, used for accurate quantitative analysis.
Image display and optimization
In software control, the image as a whole image rotation angle , Adjust the brightness, contrast and image filtering and other processing.
Go through the image comparison and pseudo color adjustment function, image quality was the most suitable.
The three-dimensional observation mode can observe any user selected gel region to three-dimensional model
The image overlay and flip function:
Superposition of multiple film observation function; film automatic turning function, users can set up turning speed; image acquisition mode, through the selection and observation of the sample screen, exposure time, get the best image.
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Easy to use
Step by step guide function the user friendly, application oriented menu interface follow analysis software, analysis mode according to the wishes of the individual, easy to obtain analytical results.
Fast application guide you from the collected image data analysis and output of the image data, without software instructions
The direct access to relevant content on the screen to help
O by clicking on the icon in the suspension inquiries on the screen, ready access to professional answers to difficult problems.
For o PC
O TIFF Input file, TIFF and JPEG Output file
O text annotations to the sample marking.
Automation Manager
O powerful Automation Manager can automatically save and run the following template function, without user intervention:
The memory lane and the position of the sample
The molecular weight determination
The quantitative labeling
The text and arrows
The advantages compared with other systems:
Clear image, good stability, simple operation, friendly interface, convenient maintenance and use of software.
Special note: before using this instrument, please carefully read the instructions. The instructions should be put together with the instrument, so that the operator to read instructions, once lost, please ask us for.
A, use:
Analysis of gel electrophoresis image for the set of laboratory equipment, using digital camera image gel is placed in the light tight box in the UV or white light irradiation taken into the computer, through the corresponding gel analysis software, can complete the analysis of RNA, DNA, protein gel, TLC plate image, we can finally get the gel with peak value, molecular weight or base pairs, area, height, position, or the total amount of sample volume.
Two, structure and characteristics
Gel imaging and analysis system is the analyzer with the black box, from ultraviolet transmission light, white light, black box, camera, computer system, gel analysis software and other components, can operate in clear room. The UV light with a white light source and three wavelength of the instrument, you can according to their own needs, using one wavelength light source alone, can also be used in several wavelength light source.
Product features:
* high sensitivity, high resolution digital imaging system;
* provide white light and UV light, the fluorescence, film, silver staining and Coomassie brilliant blue EB staining, * like digital processing;
* with gel analysis software for image analysis;
* multipurpose box, from laboratory darkroom settings, and UV or white light transmission and UV reflection photography. At the same time taking into account the design to reduce the damage of UV on human as possible;
* uniform illumination, maximum imaging area of 200 x 250 mm.
Three, the main performance index
Basic parameters
Size (L * W * H): 440 x 430 x 770mm;
UV reflection wavelength: 254nm, 365nm;
Transmission of UV light source wavelength: 300nm;
UV transmission area: 200 * 250mm.
Environmental conditions:
Ambient temperature: 5 to 40 DEG c;
Relative humidity: less than 80%;
Atmospheric pressure: 86kpa ~ 106kpa.
Power supply conditions:
Supply voltage: single-phase sinusoidal AC 220V + 22V;
Frequency: 50Hz + 1Hz.
Other：
The UV light irradiance window of each wavelength is less than 10 W/cm2;
White light is more than 100lx (Lux);
Can work continuously for 4 hours.
Digital camera can connect with the computer through the implementation of photographic imaging control, image editing processing analysis software, automatic lane identification, molecular weight calculation, the sample volume distribution calculation.
Four, installation method
A, unpacking and installation:
1. packaging inspection instruments, if there is a serious damage to inform the dealer to solve, can destroy nondestructive unpacking, should be carefully handled with unpacking process, to avoid damage to the instrument. Out of the box should have instrument control list to check all the items, please contact the dealers.
2. in the instrument power supply socket missed under the condition of power switch box, panel are placed in the off state (the power switch is switched on, the "I" "is" open). The transmission box on the tray; as with white light, directly converting plate on the transmission box.
Warning: excessive ultraviolet radiation will cause harm to the human body, so the UV light must work in the dark box, the use of prohibited exposure!
3. digital camera and lens screw, the close-up lens, filters and adapter ring are screwed in the lens, then the digital camera is fixed on the bracket at the top of the box, tighten the knob, then the power supply controller, connecting line USB lens with the computer, with the USB line of the digital camera and computer linked.
B, software installation
1) digital camera installation.
CCD connection with the computer, the following screen appears.
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Figure 5
Figure 6
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Figure 7
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Figure 8
2). Install the software
1. double click SETUP-START-OK
Figure 11
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Two take WINDOWS Copy to folder C:/ The root directory of the original coverage WINDOWS
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C, shot
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Open the scan to new document pop-up dialog box. Ver.offset numerical exposure time of 0 - 2000. Any adjustment. Cap.frames is 1. Image file. Quit shooting program. Take exit procedures at the same time each open will automatically shut down
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Figure 14
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D, image analysis
Please refer to the GEL-PRO image analysis software manual analysis
Five, matters needing attention:
1. after receiving the user please as soon as possible out of the box inspection, if not normal circumstances, please contact with my factory after receipt of the goods within a week, in order to solve.
Should be to prevent the collision, 2. earthquake in the transport process, to avoid UV glass and UV lamp damage. Should be stored in humidity of not more than 93%, the ambient temperature 0 to 55 DEG C, non corrosive gas and clean indoor ventilation.
3. of the instruments used in all metal structure, should be used to ensure the socket grounding.
4. UV light after the power is switched on the lights, the UV light must work in the dark box, the use of prohibited exposure.
A. warning: excessive ultraviolet radiation will cause harm to the human body, so the UV light must work in the dark box, the use of prohibited exposure!
B. add or replace the transmission box before, must first cut off the power supply!
5. use of such as the discovery of other anomalies should be immediately shut down, to repair. If there is a faulty instrument, please contact our factory repair.
6. UV lamp will produce brightness decay and aging in normal use, according to the frequency of use, the lamp in 3 to 12 months to replace the normal, this does not belong to the scope of warranty. Other fault free warranty for one year, after the failure, I plant is still responsible for the maintenance, reasonable fees.
If you have any questions please contact with my factory technical service center.
Six. The daily maintenance method:
1. the instrument should be placed in a cool, dry, free of dust and acid, steam, instrument use environment should be clean. Long time no should unplug the power plug and cover protective cover.
2. do not use the water and liquid splash instrument.
3. light is placed on the observed glass as a dirty, clean available alcohol cotton.
7. On the user's service commitment:
This product is used for the 4 year period. Products sold after one year warranty: one year in improper use, it is responsible for the product quality problems, repair materials, repair costs, freight collect, all free. For more than a year, is responsible for maintenance. Reasonable collection of materials, repairs.
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Eight transportation and storage.
Transport: the transport order analyzer according to the provisions of the implementation of the contract.
Storage: packaged products should be stored at the temperature of 0 DEG +55 DEG C, relative humidity not higher than 93%, non corrosive gas, good ventilation indoors.