Configuration: UV detector, high voltage constant current pump, inlet valve, 7725i Exformma series Arcus chromatographic column, WS100 chromatographic workstation software, solvent tray (according to customer demand, the configuration will be slightly different)
LC-100 liquid chromatography system is Wufeng company LC-100 series of product upgrades. "Multiple" + ", a more reliable".
LC-100 intelligent control system is composed of high pressure liquid chromatography P100 constant flow pump with UV100 UV detector structure analysis system, direct gradient analysis system. The use of WS100 workstation can also control the number of P100 high pressure constant flow pump, UV100 UV detector and temperature oven, high pressure gradient, the implementation of multi wavelength scanning function.
LC-100 intelligent control liquid chromatography system is intuitive and convenient operation. It can not contact the computer through the instrument itself small keyboard input all the control information, all operations are displayed on the LCD screen. Can also be connected to the computer, using the workstation software. Between the workstation and the chromatograph using pure digital communication, sampling data are read directly two channel, without any treatment, there is no signal distortion, so that the sampling precision and postprocessing when the data is not lost. The sampling precision is up to 18 bits (0.1 sec / *2 channel).
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WS100 workstation is divided into real-time sampling, postprocessing, wavelength playback, map comparison of four independent unit. The real-time sampling unit can directly monitor up to 4 pumps and UV detector. Can arbitrarily change the wavelength, pump flow, deuterium lamp switch, can stop the pump or the steering switch valve at any time, let the components remain in circulation in the pool, and then start the automatic wavelength scanning can be obtained, the components of continuous spectrum of different wavelengths. Can also be connected with a changeover valve through the control valve set, so as to achieve the separation and recovery of mobile phase. These are all the other workstations can not do. In addition, all display data have been converted into AU values, fold change without cumbersome user.
Because the software is running in the WINDOWS system, all the function menu, do not need to learn, one will see, simple operation
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P100 constant flow pump is a reciprocating microprocessor intelligent control parallel double plunger pump, has the characteristics of high working pressure, small pulsation, reliable and convenient operation, etc.. The mechanical components of the pump adopts computer aided design. The main parts are in the world advanced manufacturing processing center. It has high processing precision, good sealing performance, the pump body parts interchangeability good characteristics.
The plunger rod with floating guide structure, the microprocessor control circuit to drive the microstep drive, pump operation of stepper motor smooth operation, low noise, large speed regulation range. Can meet the need of routine analysis to micro flow analysis.
In the single pump analysis, according to the instrument on the whole display prompt Chinese characters LCD screen, through four function key
Can transform a variety of modes of operation, and can set the flow and pressure of convenient limits, continuous working time, the parameters such as pressure units.
P100 constant flow pump with process monitoring function. Flow control pump and microprocessor real-time detection of infusion pressure. In the case of pressure gauge, automatic alarm and stop the pump.
LC-100 high voltage constant current pump integral structure using modular design, including mechanical part and a circuit part can be independently disassembled so fault inspection and replacement are very convenient.
P100 high voltage constant current pump adopts double plunger pump parallel structure, double cross infusion pump head. In the same fluid volume, the piston rod and the sealing ring are longer than conventional double long pump series.
The WS100 workstation control up to four pumps through the RS232 interface operation, establish multi gradient analysis system.
Basic technical indicators:
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Flow range: 0.001 ~ 9.999 mL/min
In order to regulate the flow of 0.001mL/min step.
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Flow accuracy: RSD = 0.06%
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Pressure pulsation: less than 0.1 Mpa
(1mL/min flow, pressure 5 ~ 10Mpa)
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The pump seal pressure is 42MPa,
Time is 10min, the pressure drop of <0.5MPa.
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Maximum working pressure: 42MPa (0.001 ~ 9.999mL/min)
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Size: 450mm * 300mm * 160mm (length * width * height)
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? UV100 UV detector
UV100 UV detector with double hole parallel flow cell. The sample and reference pool completely under equal conditions. The flow cell cone design, can be in the same pool volume under the condition of increasing light energy passing through the cell, significantly reduced the instrument static noise and signal drift.
The optical system adopts precision positioning structure, high precision, small offset. Deuterium lamp and optical system using thermal isolation installation technology, reduces the stability time of the instrument, and the heating effect on the deuterium lamp optical system to a minimum.
The control circuit adopts multi microprocessor architecture. Management of signal acquisition, data processing, control and communication system. UV100 UV detector in doing analysis, you can easily bond with seven functions through the LCD screen full Chinese characters, set the wavelength of ultraviolet light, filtering, constant output range, running time and other parameters. And can realize the switch of deuterium lamp, spectral scanning, restart analysis function.
Another characteristic of this instrument is: UV100 UV detector circuit structure the use of unique digital signal output directly, avoiding the chromatographic signal of UV detector of the general signal distortion and interference by the analog-to-digital converter and the multiple.
UV100 UV detector can through the graphical interface on the LC-100 workstation, set the parameters to facilitate the realization of the control of deuterium lamp switch, relay switch, wavelength scanning, scanning and other functions.
Basic technical indicators:
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Wavelength range: 190 ~ 680nm
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The baseline noise is less than or equal to + 0.25 * 10 -5 AU (empty pool)
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The spectral bandwidth: 8nm
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Baseline drift: less than 0.4 * 10 -4 AU (empty pool)
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The value of error is less than or equal to wavelength is shown . 0.5nm
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The minimum detection amount: 1 * 10 -8 G/mL (methanol solution of naphthalene)
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Wavelength repeatability is better than 0.2nm
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Size: 450mm * 300mm * 160mm (length * width * height)
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WS100 chromatography workstation
The WS100 workstation software to realize the automatic integration of UV detector and high pressure constant flow pump, has strong control function and simple operation.
Function introduction
This software adopts numerical control system, digital operation makes the process more simple, and has high accuracy. The software consists of two modules, namely control LC-100 liquid chromatography chromatography module and data processing module.
Control LC-100 liquid chromatography system:
Through the RS232 communication cable between UV100 and UV detector and UV100 UV detector with P100 high voltage constant current RS485 communication cable between the pump can be up to four P100 high voltage constant current pump and control under a graphical interface, and UV100 UV detector to achieve multiple pressure gradient elution, wavelength scanning, spectrum scanning and relay switching events and other functions, and can display multiple pump flow time curve and wavelength scanning curve.
The main functions are as follows:
Gradient elution: users only need to input the proportion of mobile phase in the corresponding list, flow and change of time, can realize the easy operation of the accurate gradient elution. The software also provides the corresponding pump flow curve, which is convenient for the user to proofread and enable the user to set the gradient, a more intuitive understanding of the gradient process.
In order to test the spectral scanning: deuterium lamp, software provides a spectral scanning function. First, the user can directly detect to the size of deuterium lamp intensity under different wavelength, and can judge whether you need to replace deuterium lamp. It can also be through the 486.6nm and 656nm of the two position to judge the accuracy of wavelength error.
Wavelength scanning: wavelength scanning function software provided in the range of 180 - 680nm, minimum step size is 1nm, which can help users understand the absorption of samples of different wavelengths of ultraviolet light, so that users in different samples with different wavelength detection to achieve the best effect analysis.
Event: the time relay control program event relay, we can realize all kinds of users, such as steering switch valve to achieve the separation and recovery of mobile phase. The function of popular workstation can be realized.
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Chromatography data processing: receives the digital signal sent by UV100 UV detector, direct data processing. The basic functions of:
6 calculation methods: quantitative normalization method, correction normalization method, correction normalization method, the scale factor of internal standard method, external standard method, calculation method and index.
The calibration operation: to achieve a variety of different concentrations of standard samples for calibration, sample concentration area correction curve.
Peak recognition and flexibility: can handle by setting parameters of peak and the recognition time of chromatographic peaks and processing program, can also manually processed.
Spectrum finishing: chromatography graphics, set quantitative calculation, peak processing parameters, peak identification table can be saved to the file name from the user. Since the LC- 100 chromatography workstation receives the sample pool sent UV detector and reference pool light intensity real-time digital signals on the workstation preserve the original data information, there is no signal distortion caused by multiple signal processing, so the chromatographic signal postprocessing work without any restrictions. Due to the realization of real-time data processing and control, to the specified peak detection in the analysis process can automatically stop the switching operation or control of pump valve, the specified components remain in circulation in the pool, and then automatically start the scanning wavelength component spectra of different wavelength absorption rate.
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Simple and fast operation due to workstation to chromatographic systems real-time control and data processing modules are in the Chinese WINDOWS operating system, provides the interface and menu selection function of graphical cursor and stay prompt function, the operation is convenient, flexible switch. As long as a basic knowledge of computer operation chromatographic analysis personnel, without training, you can use.
Simple interface settings: software main interface includes basic settings for all UV detector and high voltage constant current pump, users only need to click the mouse button and keyboard can be directly input. The user can at any time according to need to control the deuterium lamp switch, zero return real-time sampling data, adjust the filtering time and wavelength setting, but also in the operation of the event and the corresponding set enable wavelength scanning procedures. The user can also control the opening and stop the pump at any time, and the corresponding set of pressure limit and instant flow.
Set clear parameters: convenient in parameter settings in the dialog box, users only need to input different parameters directly according to the required value can be determined to set the parameters of peak processing parameters and the implementation of the system.
One step conversion: WS100 workstation has chromatographic data processing parameters are converted into AU value and absorption rate of the unit, users do not need complicated conversion.