a An overview of the

RISTRON@ Microfluidic chip temperature perfusion apparatus by yongchuan affiliated hospital of chongqing medical university and Shanghai chi tang industrial co., LTD., mainly used in all kinds of cytological experiments on the microfluidic chip.Trace liquid control system and the system integration of precision constant temperature transparent microscope platform, through the computer precision control of the liquid flow, velocity, time and temperature, outside the incubator to reshape cells growth environment conditions, suitable for cell perfusion culture and on the microfluidic chip analysis, combined with the conventional inverted microscope, achieve the function of cell culture implementation of monitoring and analysis.

Product main characteristics:

n Precise control of liquid flow rate and temperature for incubator irrigation fluid outside the cell culture provide the right conditions, microfluidic chip, a cell continuous perfusion culture3Days or more;

n You need to write according to the experiment tasks (each task can be set8A step), each step can be precise control flow, velocity, time and temperature, is advantageous for the realization of microfluidic chip pretreatment and automation cell culture, and switching operation;

N culture, at the same time can be related to the dosing, dyeing processing, can achieve cell function analysis;

N with inverted microscope, the realization of real-time dynamic monitoring imaging cells for a long time;

N heating thermostatic platform area is large and uniform temperature distribution, suitable for conventional petri dish constant temperature, also suitable for many pieces of microfluidic chip parallel experiments.

The second Instructions for

2.1 the instrument hardware connection

willStandard power supply 24 v DC power supply 24 interface connected with the master to instrument power supply;Thermostatic plate with white telecommunication lines connect host and constant temperature plate;Use USB line control computer connected to the host USB interface.Through the power switch on or off instruments;

figure2.1 Instrument hardware interfaces and instrument parts name

2.2 syringe installation

Step 1: complete instrument hardware connection, open the power switch startup system;

Step 2: start the software control system, into the components testing interface;

Step 3: left or right piston feed mechanism, is advantageous for the syringe installation;

Step 4: let go of piston and syringe fluctuation pressure clamping knob knob, install the syringe, tighten the piston and syringes fluctuation pressure clamping knob knob, pressure injector sleeve, as shown in figure2.2;

figure2.2 Syringe installation schematic diagram

2.3 driver installation

withUSB cable connected the instrument computer USB host and control;Open the instrument on the right side of the power switch, instrument power indicator light on the host;Use need to install the USB driver for the first time, in the companion cd-rom into the folder "USB to serial driver", "PL2303 Windows driver v150" into the folder, double-click the PL2303_Prolific_DriverInstaller_v1. 5.0. Exe, can complete the instrument USB driver installation;Computer after the installation is complete will figure 2.3 interface, instrument USB driver software is successfully installed, you can use;

figure2.3 The instrumentUSBThe driver is successfully installed

2.4 software instructions

Start against 2.4.1 software

In the form a complete set of CD"Running software" folder, double-click the CellSoft_Anron. Exe start instrument control software, such as instrument and computer communication is normal, instrument communication indicator light on the host, start software interface prompt "Device Initial Success!",

There are four buttons below the instrument control software startup interface: component testing, parameter Settings, main control interface, and copyright information.

2.4.2 component test

Click on the component test pageAnd into the components testing interface, as shown in figure2.4.2.

figure2.4.2 Control software component testing interface

(1) injection pump volume setting: syringe range(uL) as the experimental model is set with a syringe, input using the standard syringe stroke (1 ml and 5 ml, 10 ml), etc., which need to input a value of (1000, 5000100, 00), click to confirm changes, complete the syringe set selection;

（2) test injection pump parts: used to syringe set left and right limit range, so as not to damage the syringe or fall off the syringe putter to liquid falls;Users can input speed and mobile distance, at the same time the interface displays the current position;Click on the Shift to the left Move to the left button motor, namely liquid injector;Click on theMoves to the right Button machine to the right, namely the syringe liquid;Each move speed and distance for user input values;When motor moves to a position, click set left limit or set the right limit, the system will be in the current position as a left limit or right limit, and shows the location of the two limit;It is important to note that in order to better protect the syringe is not damaged, the user in setting up the left limit, suggested that the piston not greater than syringe biggest moment which affects;In setting up the right limit, piston and"0" scale line position in a 4-5 mm.

Set completion of left and right limit, clickable moved to left limit position and moved to the right limit position can test whether the syringe in setting up the left and right limit position stop, in order to ensure the safety operation;

（3) the LED test: this part is mainly to test the host LED indicator light is normal display and control and host computer communication is normal, the selected is bright, is not selected is not bright (except the power light);

（4) temperature control unit test: this part is mainly to test instrument constant temperature board is working correctly, without need for liquid, the part can be simply used as a constant temperature microscope platform;Users can set temperature degree, (default) 30 degrees, click on the temperature control in temperature control mode, click the stop temperature controlThe temperature control to stop; If you check theReal-time read Automatically read the current temperature and display, can be manuallyGet the current temperature;

2.4.3 parameter Settings

Click the start interface parameter Settings, enter the parameter Settings interface, as shown in figure2.4.3.

figure2.4.3Control parameter Settings interface software

The new button to add new tasks;Delete button to delete the currently selected tasks;The modify button to modify the current task;The save button to save the current task.

A new task:Click newButton, the corresponding text box input task name, task steps respectively;Add the step name, traffic(uL) on behalf of the operation by the liquid volume this step;The length (s) on behalf of the complete set the liquid flow operation time;Starting speed (uL/s) and the initial flow (uL) respectively before operation set liquid flow operation (mainly used in micro fluid quantitative existing in the operation of pipeline fluid road compensation volume).Temperature (℃) on behalf of the board of performing this step temperature control temperature;Wait time (s) on behalf of the execution of the steps to the time interval to take the next step;Check the push fluid said the steps push fluid operation, liquid does not check the steps.

Delete the task:Choose in the task bar needs to delete task, click the delete button, delete the selected task.

Modify the task:Choose in the task bar needs to delete task, click on the modify button, can be the name of the task, the number of steps, and each step parameters changes;

Save the task: to complete tasks modified, click on the save button, save to do the job;

2.4.4 master interface

Choose the need to perform tasks in the parameter Settings screen, click on the main control interfaceButton, carries on the control software of main control interface, as shown in figure2.4.4.

figure2.4.4The main control interface of control software

Click the start button, instrument began to perform a task;Click the pause button, task execution, pause button change to continue button, click continue to run the task from the current step;Click the stop button, interruption of the current task, need to click the start button, can to perform a task.

Click on a step, or the next step button, namely to skip the current task steps, run this step is a step or the next step;

After completion of task to run or task to stop, click the left or the right limit limit button syringe will return to set left limit or right limit;

The current temperature of real-time display temperature plate temperature, at the same time, the master interface surface also showed temperature curve and task the information of each step.

2.4.5 copyright information

Click the copyright information button, display device developer's name and contact information.

three System parameters

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